Journal: Biosensors and Bioelectronics X
Article Title: Aeromonas veronii specific aptamer and peroxidase mimic tyrosine-capped gold NanoZymes enable highly specific sensing of fish pathogenic bacteria
doi: 10.1016/j.biosx.2024.100505
Figure Lengend Snippet: Scheme 1. The schematic depicts the steps required in A. veronii sensing. The approach begins by assessing the peroxidase-mimic catalytic activity (NanoZyme) of AuNPs which transforms the TMB enzyme substrate into blue colored product (NanoZyme ON). After incubating the AuNPs with A. veronii aptamers, an aptamer- AuNPs conjugate (sensing probe) is generated, in which the aptamers adsorbed the surface of the AuNPs via noncovalent interactions, resulting in a loss of catalytic activity (NanoZyme OFF). Once A. veronii is exposed to the sensor probe, the aptamer’s strong affinity for A. veronii causes aptamer desorption from the AuNPs, resulting in catalytic activity recovery (NanoZyme ON). Nevertheless, no color appears when the sensor probe is exposed to other bacteria, suggesting that the aptamers do not desorb from the surface of the AuNPs (NanoZyme Off).
Article Snippet: The target fish pathogenic bacteria A. veronii (AV) and other bacterial pure cultures (A. hydrophila (AH), P. aeruginosa (PA), Klebsiella pneumoniae (KP), and Bacillus sp.) were isolated from diseased fish (Labeo rohita) collected from an aquaculture farm in West Bengal.
Techniques: Activity Assay, Generated, Bacteria